Effect of Activators and Inhibitors on Lactase Activities to Determine its Kinetic Model

نویسنده

  • R. Madani
چکیده

Lactase activity in presence of mono and divalent metal ions, as its cofactors, and some reducing agents was studied. Activation effects of k and Mn were better than other cations and dithiotrethiol was more inhibitor. By inhibition constant, 12.15mMol, the type of inhibition was non-competitive. The kinetic model of Lactase hydrolysis reaction also was determined. Among glucose and galactose as its products, galactose had more inhibition effect and the type of inhibition was non-competitive. Galactose inhibition constant in lactose substrate was 78.7mMol.

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تاریخ انتشار 2006